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  • Title: The reactivity of 4,5',8-trimethylpsoralen with oligonucleotides containing AT sites.
    Author: Ramaswamy M, Yeung AT.
    Journal: Biochemistry; 1994 May 10; 33(18):5411-3. PubMed ID: 8180164.
    Abstract:
    Pyrimidine bases of duplex DNA, of appropriate sequence context, are photoreactive toward 4,5',8-trimethylpsoralen in the presence of long-wavelength UV light. It is generally believed that a 5'-AT site is less photoreactive with psoralen than a 5'-TA site. We have compared the reactivities of these two sites using oligonucleotide duplexes of different sequence context and found that 5'-TA and 5'-AT sites are equally reactive in certain sequences. The presence of alternating pyrimidine and purine (5'-PyATPu-3') bases in oligonucleotide duplexes optimizes the reactivity of 4,5',8-trimethylpsoralen in the 5'-AT sites.
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