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  • Title: Structures of small oligomers liberated from barley arabinoxylans by endoxylanase from Aspergillus awamori.
    Author: Viëtor RJ, Hoffmann RA, Angelino SA, Voragen AG, Kamerling JP, Vliegenthart JF.
    Journal: Carbohydr Res; 1994 Feb 17; 254():245-55. PubMed ID: 8180986.
    Abstract:
    Arabinoxylans consisting of more than 90% of arabinose and xylose were extracted from water-insoluble cell wall material of dehusked barley grain, and degraded with a purified endo-(1-->4)-beta-D-xylanase from Aspergillus awamori. Twelve of the oligosaccharide fragments released were isolated by a combination of size-exclusion and anion-exchange chromatography, and their structures were determined by 1H NMR spectroscopy. The oligosaccharides identified consisted of (1-->4)-linked beta-D-xylopyranosyl residues, some of which were substituted at O-2, at O-3, or at both O-2 and O-3, with alpha-L-arabinofuranosyl groups. Two new structures, a tetrasaccharide and a pentasaccharide, both containing terminal 2-O-arabinofuranosylxylopyranose as a structural element are described.
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