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  • Title: Characterization of a Mycoplasma hominis gene encoding lysyl-tRNA synthetase (LysRS).
    Author: Ozkökmen D, Birkelund S, Christiansen G.
    Journal: FEMS Microbiol Lett; 1994 Mar 01; 116(3):277-82. PubMed ID: 8181699.
    Abstract:
    The gene encoding lysyl-tRNA synthetase (lysS) in Mycoplasma hominis was cloned and sequenced. The gene was found to have an open reading frame of 1466 bp encoding a polypeptide with a predicted molecular mass of 57 kDa. The amino acid sequence showed 44.3% and 43.7% identity to the Escherichia coli lysyl-tRNA synthetases, encoded by lysS and lysU. Only one lysyl-tRNA synthetase encoding gene was found in M. hominis. The G + C content of the gene was found to be 28.6%, which is significantly lower than in other prokaryotes. The gene was located 4 kb upstream of the M. hominis PG21 rRNA B operon.
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