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  • Title: Thiostrepton-resistant mutants of Bacillus subtilis: localization of resistance to the 50S subunit.
    Author: Pestka S, Weiss D, Vince R, Wienen B, Stöffler G, Smith I.
    Journal: Mol Gen Genet; 1976 Mar 30; 144(3):235-41. PubMed ID: 818503.
    Abstract:
    A number of thiostrepton-resistant mutants of Bacillus subtilis were obtained. The thi mutations map proximally to strA. Effects of thiostrepton on polyphenylalanine synthesis with ribosomes of S-100 fractions from parent and mutant strains indicated that resistance was localized to the ribosomes. Furthermore, effects of thiostrepton on binding of [3H]GTP to ribosomes and 50S subunits from thiostrepton-sensitive and -resistant strains localized the site of resistance to the 50S subunit. In addition, revertants from thiostrepton-resistance to thiostrepton-sensitivity were obtained. Ribosomes and 50S subunits from these thiostrepton-sensitive revertants were sensitive to thiostrepton similar to parental sensitive B. subtilis.
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