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  • Title: [The relationship between vaccinia virus DNA replication and intermediate filaments].
    Author: Yu WD, Yu Q, Ding MX, Zhai ZH.
    Journal: Shi Yan Sheng Wu Xue Bao; 1993 Sep; 26(3):239-47. PubMed ID: 8191801.
    Abstract:
    Various DNA components which were extracted with gentle cell fractionation from the HeLa cells after 4 h vaccinia virus infection were detected by dot hybridization technique. The virus DNA mainly exist in intermediate filament-lamina-nuclear matrix complex. With DGD embedment free technique and electron microscopic autoradiography, the newly synthesized virus DNA is found to be associated with intermediate filaments. The results of southwestern hybridization demonstrate that vaccinia virus DNA has specific affinity to intermediate filaments and some nuclear matrix proteins.
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