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  • Title: Determination of free myo-inositol in milk and infant formula by high-performance liquid chromatography.
    Author: Indyk HE, Woollard DC.
    Journal: Analyst; 1994 Mar; 119(3):397-402. PubMed ID: 8192226.
    Abstract:
    A high-performance liquid chromatographic method is described for the determination of supplemental myo-inositol in infant formulas based on cows' milk. The technique incorporates pre-column derivatization with phenylisocyanate followed by reversed-phase gradient chromatography and ultraviolet detection. The protocol was also applied to a survey of free inositol at endogenous levels in the milk of cows, goats and humans. Nutritional preparations containing inositol at pharmacological levels are also amenable to a simplified isocratic analysis.
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