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  • Title: A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium.
    Author: Gessner M, Raeder U.
    Journal: Gene; 1994 May 16; 142(2):237-41. PubMed ID: 8194757.
    Abstract:
    In this study, two transformation vectors (pMG101 and pMG103) for Phanerochaete chrysosporium were constructed, based on the ble phleomycin-resistance-encoding gene and a homologous histone H4 promoter. Transformation frequencies were 6-10 per micrograms of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of ble-dependent resistance. Plasmid pMG103, which lacks the H3, does not show methylation.
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