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  • Title: Developmentally regulated oligosaccharides in mouse spermatogenic cells.
    Author: Maylie-Pfenninger MF.
    Journal: Arch Biochem Biophys; 1994 Jun; 311(2):469-79. PubMed ID: 8203912.
    Abstract:
    Mouse spermatogenic cells were separated into four populations, pachytene spermatocytes, round spermatids, elongated spermatids, and residual bodies. Each cell population was metabolically labeled with [3H]galactose, [3H]glucosamine, or [3H]fucose. Glycopeptides were prepared from the radiolabeled glycoproteins by pronase digestion and fractionated by serial lectin affinity chromatography and gel filtration. The presence of O-linked oligosaccharides was assessed by pronase digestion of [3H]galactose-labeled glycoproteins, exclusion of the radiolabeled glycopeptides from a ConA-Sepharose column, gel filtration, and treatment with alkaline borohydride. This analysis reveals that a large proportion of [3H]galactose-labeled oligosaccharides (47-52%) are O-linked structures, while the majority (80-90%) of [3H]fucose-labeled oligosaccharides are N-linked. The proportions of triantennary, biantennary, oligomannose, and hybrid oligosaccharides linked to asparagine vary with the cell populations. Furthermore, in round spermatids, but not in the other cell populations, a relatively large proportion (15%) of [3H]glucosamine-labeled oligosaccharides consists of terminal O-linked N-acetylglucosamine. Taken together these data show that each spermatogenic cell population contains a unique complement of oligosaccharide structures that could play an important role as differentiation signals in the interactions among these cells and/or with Sertoli cells.
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