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  • Title: Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.
    Author: Opstelten DJ, Horzinek MC, Rottier PJ.
    Journal: Adv Exp Med Biol; 1993; 342():189-95. PubMed ID: 8209729.
    Abstract:
    Using different approaches we have demonstrated the formation of a complex between the S protein and the M protein in the process of mouse hepatitis virus (MHV) assembly. Preservation of the M/S heterocomplexes was critically dependent on the solubilization conditions. Pulse-chase labeling of MHV-infected cells followed by a co-immunoprecipitation assay revealed that newly synthesized S and M engage in complex formation with different kinetics, the S protein reacting much slower. Sedimentation experiments showed the M/S heteromultimer complexes to be very large. A model for the role of the complex formation in MHV assembly is presented.
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