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  • Title: Hydroxylation of the native brassinosteroids 24-epicastasterone and 24-epibrassinolide by the fungus Cunninghamella echinulata.
    Author: Voigt B, Porzel A, Naumann H, Hörhold-Schubert C, Adam G.
    Journal: Steroids; 1993 Jul; 58(7):320-3. PubMed ID: 8212079.
    Abstract:
    24-Epicastasterone and 24-epibrassinolide, two naturally occurring phytohormones of the brassinosteroid type, were transformed by the fungus Cunninghamella echinulata to give the corresponding 12 beta-hydroxylated compounds. The structures of these compounds were determined by spectroscopic methods, especially heterocorrelated two-dimensional nuclear magnetic resonance investigations. In the rice lamina inclination test the 12 beta-hydroxylation lowered the bioactivity at 0.1 ppm to 10% in comparison with that of the corresponding parent hormones. The described hydroxylations represent the first biotransformations of native brassinosteroids.
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