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  • Title: The bcl-2 protein is inserted into the outer membrane but not into the inner membrane of rat liver mitochondria in vitro.
    Author: Nakai M, Takeda A, Cleary ML, Endo T.
    Journal: Biochem Biophys Res Commun; 1993 Oct 15; 196(1):233-9. PubMed ID: 8216296.
    Abstract:
    The bcl-2 gene encodes a 26 kDa protein, overexpression of which blocks cell apoptosis. Since conflicting data have been reported concerning the localization of the Bcl-2 protein with mitochondrial inner and outer membranes, we analyzed in vitro import of the human Bcl-2 protein into isolated rat liver mitochondria. The Bcl-2 protein translated in rabbit reticulocyte lysate was efficiently inserted into the mitochondrial outer rather than the inner membrane in a membrane potential-independent manner. The carboxyl-terminal hydrophobic stretch is essential for the Bcl-2 protein to integrate into the outer membrane. Binding of the Bcl-2 protein to the mitochondria was not affected by pretreatment of the mitochondria with trypsin, indicating that the Bcl-2 protein does not require protease-sensitive mitochondrial surface components for its membrane insertion.
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