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  • Title: A rapid, automated microtiter assay for measuring neutralization of HIV-1.
    Author: Laal S, Burda S, Sharpe S, Zolla-Pazner S.
    Journal: AIDS Res Hum Retroviruses; 1993 Aug; 9(8):781-5. PubMed ID: 8217345.
    Abstract:
    A sensitive, rapid, and quantitative ELISA for p24 is described, which can be used as the read-out to test for HIV-1 neutralization in the syncytium-forming microassay and can replace the counting of syncytia under the microscope. This assay can be used reliably for divergent strains of HIV-1, including those that do not induce syncytia. The new read-out permits evaluation of neutralization at 3 days rather than 5 days. Using this assay, the neutralizing activity of several new and previously described human monoclonal antibodies against HIV-1 was characterized.
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