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  • Title: Fate of thyrotropin releasing hormone after binding and stimulation of prolactin release by GH3 cells. Evidence for release of unmodified (3H)-TRH.
    Author: Gourdji D, Tixier-Vidal A, Levine-Pinto H, Pradelles P, Morgat JL, Fromageot P.
    Journal: Neuroendocrinology; 1976; 20(3):201-11. PubMed ID: 822368.
    Abstract:
    GH3 cells which had been exposed for 30 min to (3H)-TRH and had performed their maximun biological response, i.e. increase of prolactin (PRL) release, retained intracellular radioactivity which consisted of chemically unmodified TRH (greater than or equal to 93%). Such preloaded GH3 cells were found able to spontaneously release into the culture medium a radioactive material (3H)-RM) which was analyzed. The kinetics of binding of (3H)-RM to intact GH3 cells and competition with unlabeled TRH were indistinguishable from (3H-TRH. (3H)-RM was able to stimulate PRL release from GH3 cells. In addition thin layer electrophoresis (TLE) revelaed that 90% of (3H)-RM migrated like TRH reference. All these features strongly suggest that (3H)-RM is identical to (3H)-TRH.
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