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  • Title: Trimeric mutant bacteriorhodopsin, D85N, shows a monophasic CD spectrum.
    Author: Kataoka M, Mihara K, Kamikubo H, Needleman R, Lanyi JK, Tokunaga F.
    Journal: FEBS Lett; 1993 Oct 25; 333(1-2):111-3. PubMed ID: 8224146.
    Abstract:
    The structure of mutant bacteriorhodopsin (bR), D85N, was examined by CD and X-ray diffraction at pH 7. The absorption maximum of D85N at pH 7 is located at 605 nm, which is similar to the acid-blue form of wild-type bR. D85N shows a monophasic CD band, the maximum of which is at 575 nm, although the crystalline arrangement and the trimeric structure is maintained. The acid-blue form of wild-type bR shows a biphasic CD despite the similarity in absorption spectra.
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