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  • Title: rab3-peptide stimulates exocytosis from mast cells via a pertussis toxin-sensitive mechanism.
    Author: Law GJ, Northrop AJ, Mason WT.
    Journal: FEBS Lett; 1993 Oct 25; 333(1-2):56-60. PubMed ID: 8224171.
    Abstract:
    Synthetic peptides of the putative effector domain of members of the rab3 gene family of small GTP-binding proteins have been shown to have potent actions on vesicular transport and exocytosis [1,2]. Here, we use similar rab3-effector domain peptides to study their role in intracellular signalling in mast cells. We find that rab3-like peptides stimulate exocytosis and decrease cyclic 3',5'-AMP levels in these cells when applied extracellularly. Cells pretreated with pertussis toxin (PtX) to selectively uncouple alpha i/alpha o type G proteins from their biological activators, however, did not respond to rab3 peptides. rab3-like peptides also induce a Ca2+ transient in mast cells. These observations provide evidence for functional coupling between an effector domain peptide sequence of rab3 protein and a PtX-sensitive G protein substrate.
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