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Title: Insertion of transposon Tn5seq1 into G+C-rich DNA of Streptomyces avermitilis: generation of 8-, 9-, and 10-bp duplications. Author: Occi JL, Gibbons PH, Wong E, MacNeil DJ. Journal: Plasmid; 1993 Sep; 30(2):167-9. PubMed ID: 8234491. Abstract: The sites for 24 Tn5seq1 insertions into G+C-rich DNA of Streptomyces avermitilis were determined. Although Tn5-based transposons usually generate a 9-bp duplication at their insertion site, one Tn5seq1 insertion generated a 10-bp duplication and another insertion an 8-bp duplication.[Abstract] [Full Text] [Related] [New Search]