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  • Title: Transformation of Mycoplasma pneumoniae with Tn4001 by electroporation.
    Author: Hedreyda CT, Lee KK, Krause DC.
    Journal: Plasmid; 1993 Sep; 30(2):170-5. PubMed ID: 8234492.
    Abstract:
    Mycoplasma pneumoniae was transformed with the Staphylococcus aureus transposon Tn4001 by electroporation. A transformation frequency of 10(-3) to 10(-5)/colony-forming unit was observed using 30.0 microgram plasmid DNA and 10(7)-10(8) M. pneumoniae colony-forming units. DNA hybridization analyses using standard and pulsed field agarose gel electrophoresis confirmed chromosomal insertion of the transposon, apparently by a transpositional mechanism into random sites. These studies demonstrate the functionality of Tn4001 in M. pneumoniae and suggest its potential as a genetic tool in this mycoplasma.
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