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  • Title: A quantitative determination of singlet oxygen with horseradish peroxidase.
    Author: Kreitner M, Alth G, Koren H, Loew S, Ebermann R.
    Journal: Anal Biochem; 1993 Aug 15; 213(1):63-7. PubMed ID: 8238883.
    Abstract:
    A singlet oxygen determination method based on the formation of dimethyl-(2,5)-2-methoxy-5-hydroperoxydihydrofuran (DMFO2) followed by acid hydrolysis with 0.1 N H2SO4 is described. Hydrogen peroxide is formed thereby as a product of hydrolysis which is then determined by the horseradish peroxidase-catalyzed formation of the homovanillic acid dimer. Since DMFO2 is a crystalline compound with sufficient stability it can be used as a primary standard for the determination. The peroxidase-catalyzed hydrogen peroxide determination allows detection of singlet oxygen concentrations as low as 10(-7) M.
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