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  • Title: Time course changes in cytoskeletal structures of cultured endothelial cells exposed to shear stress.
    Author: Ookawa K, Sato M, Ohshima N.
    Journal: Front Med Biol Eng; 1993; 5(2):121-5. PubMed ID: 8241028.
    Abstract:
    Changes in patterns of microfilament distribution were analyzed using cultured porcine aortic endothelial cells under shear flow. Within the elongated endothelial cells after shear flow exposure (20 dyne/cm2, 0-24 h), F-actin filaments were mainly observed as stress fibers. After 24 h exposure, the fluorescence intensity of rhodamine-phalloidin-stained cells was enhanced, indicating the increase in F-actin content. It was suggested that not only F-actin distribution but also the content may be affected by shear stress at the early stage of exposure.
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