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  • Title: Molecular cloning, sequencing and expression of core and NS3 fragments of HCV from patients with HCV infection.
    Author: Tan DM, Hu GL, Arima T, Zhang Z, Nanba T, Hatanaka T.
    Journal: Chin Med J (Engl); 1993 Jul; 106(7):522-6. PubMed ID: 8243124.
    Abstract:
    Fragments of core and NS3 of hepatitis C virus-Hunan (HCV-Hun) were cloned by RT-PCR and gene recombinant techniques from blood samples collected in Hunan Province, China. In comparison with sequences of our samples with those of HCV-US and HCV-J, the homologies of nucleotides and amino acids were about 90%, indicating that fragments of core and NS3 of HCV-Hun were in a relative conserved region of HCV. Two fusion proteins containing the peptides coded by HCV core (MBP-HCV core) and HCV. NS3 (MBP-HCV. NS3-Gal) were expressed by Escherichia Coli with recombinant plasmids. The specific HCV antigenicity of the two fusion proteins were identified by western blotting. Therefore, MBP-HCV. core and MBP-HCV.NS3-Gal were found useful for anti-HCV assay.
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