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  • Title: Raf-1 forms a stable complex with Mek1 and activates Mek1 by serine phosphorylation.
    Author: Huang W, Alessandrini A, Crews CM, Erikson RL.
    Journal: Proc Natl Acad Sci U S A; 1993 Dec 01; 90(23):10947-51. PubMed ID: 8248196.
    Abstract:
    Recombinant Mek1 and Raf-1 proteins produced in Sf9 cells undergo a tight association both in vivo and in vitro, which apparently does not depend on additional factors or the kinase activity of Mek1 or Raf-1. The complex can be disrupted by two polyclonal antibodies raised against Raf-1 peptides. Coinfection with Raf-1 activates Mek1 > 150-fold, and coinfection with Raf-1 and Mek1 activates Erk1 approximately 90-fold. The activation of Mek1 by Raf-1 involves only serine phosphorylation, which is directly proportional to the extent of Mek1 activation. Phosphopeptide maps suggest a single Raf-1 phosphorylation site on mek1.
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