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  • Title: [Estimation of free calcium level within synaptosomes by using fura-2 and the effect of calcium channel agonist and antagonist].
    Author: Gao H, Feng YP.
    Journal: Yao Xue Xue Bao; 1993; 28(6):404-9. PubMed ID: 8249597.
    Abstract:
    The cytosolic free calcium concentration [Ca2+]i in synaptosomes was determined with the fluorescent indicator fura-2, the effects of calcium channel agonist and antagonist on intracellular Ca2+ level were studied. The cytosolic ionized calcium concentrations in resting status were between 200 nmol/L and 400 nmol/L. Cytosolic Ca2+ was elevated following increases in Ca2+ concentration in the medium, plasma membrane depolarizations induced by KCl, and the addition of glutamate and NMDA. On the other hand, the increase of cytosolic Ca2+ induced by KCl was decreased by verapamil and that induced by NMDA was decreased by MgCl2. A few critical problems in [Ca2+]i detection were also discussed.
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