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  • Title: Simultaneous determination of creatine compounds and adenine nucleotides in myocardial tissue by high-performance liquid chromatography.
    Author: Teerlink T, Hennekes M, Bussemaker J, Groeneveld J.
    Journal: Anal Biochem; 1993 Oct; 214(1):278-83. PubMed ID: 8250235.
    Abstract:
    A rapid high-performance liquid chromatography method was developed for the determination of creatine phosphate, creatine, adenine nucleotides, and related compounds in myocardial tissue. Analysis was performed by reversed-phase chromatography on a C18 column containing 3-microns particles, employing gradient elution and uv detection at 210 nm. Separation was achieved in less than 5 min. Total analysis time, including equilibration of the column after return of the gradient to starting conditions, was 8 min. The high reproducibility and short analysis time make this method suitable for the routine analysis of large series of samples.
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