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  • Title: Efficient gene transfer with less cytotoxicity by means of cationic multilamellar liposomes.
    Author: Yagi K, Noda H, Kurono M, Ohishi N.
    Journal: Biochem Biophys Res Commun; 1993 Nov 15; 196(3):1042-8. PubMed ID: 8250862.
    Abstract:
    A simple procedure for the preparation of cationic multilamellar vesicles (MLV) consisting of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine in a molar ratio of 1:2:2 was devised. When bacteriophage lambda DNA was encapsulated into these liposomes, entrapment efficiency was found to be nearly 100%, and digestibility of the DNA was less than 10%. Upon encapsulation of the plasmid pCH110 into cationic MLV, efficient expression was comparable to that obtained with cationic vesicles prepared by reverse-phase evaporation method (REV). Cytotoxicity of the present liposomes was less than that of cationic REV and far less than that of Lipofectin.
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