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  • Title: [A general method for the determination of the conformation of nucleotides bound to an enzyme site. Interaction between glycogen phsophorylase b and nucleoside-5'-monophosphates].
    Author: Morange M, Kolb A, Buc H, Chachaty C, Langlet G.
    Journal: C R Acad Hebd Seances Acad Sci D; 1976 Nov 08; 283(11 D):1341-4. PubMed ID: 825244.
    Abstract:
    The conformation of 5' nucleotides in the active site of glycogen phosphorylase b has been deduced from T2 relaxation time of protons H1, H8 and H2. It is shown by deuterium substitution of the purine ring in position 8 that the orientation of the base is anti in the case of strong activators like adenosine 5'-monophosphate, and syn in that of weak activators like inosine 5'-monophosphate.
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