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  • Title: Detection and localization of HIV RNA and TNF mRNA in rectal biopsies from patients with AIDS.
    Author: Reka S, Kotler DP.
    Journal: Cytokine; 1993 Jul; 5(4):305-8. PubMed ID: 8260595.
    Abstract:
    The objective of this study was to compare the localization of cells containing human immunodeficiency virus (HIV) RNA and tumor necrosis factor (TNF)-alpha mRNA in rectal mucosa by RNA in-situ hybridization in a retrospective analysis of archived rectal biopsy specimens. RNA in-situ hybridization studies were performed in 27 HIV-seropositive individuals and seven controls, using antisense and sense 35S-labeled riboprobes. The detection and localization of positive cells were compared. HIV was RNA detected in 44% of biopsies, while TNF-alpha mRNA detected in 22%. TNF mRNA was found in biopsies from patients with and without opportunistic infections. All cells expressing TNF-alpha mRNA and most of the cells expressing HIV RNA were found in close proximity to the epithelial surface. The content of an HIV-associated protein, p24, in mucosal homogenates, determined by a quantitative ELISA technique was significantly higher in the subgroup of patients with positive in situ hybridization studies for TNF-alpha mRNA than in the subgroup with negative studies. The colocalization of TNF-alpha mRNA and HIV RNA immediately beneath the epithelium suggests a specific relationship between them, as well as a possible relationship to a luminal factor.
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