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  • Title: Serum-free conditioned medium of human diploid fibroblast cells contains an activity that stimulates prostacyclin production by cultured bovine aortic endothelial cells.
    Author: Yamauchi T, Umeda F, Masakado M, Ono Y, Nawata H.
    Journal: Biochem Mol Biol Int; 1993 Sep; 31(1):65-71. PubMed ID: 8260947.
    Abstract:
    Serum-free culture medium conditioned by human diploid fibroblast cells stimulated prostacyclin production by cultured bovine aortic endothelial cells. Gel filtration chromatography using Sephacryl S-100HR revealed at least three peaks of prostacyclin-stimulating activity. This factor was relatively heat-stable, acid-labile, trypsin-sensitive and bound to heparin Sepharose CL-6B. This factor was completely inhibited by indomethacin.
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