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  • Title: [Immunhistochemical investigations for evidence of insulin, glucagon and gastrin in the islets of Langerhans of Wistar rats during the development (author's transl)].
    Author: Dorn A, Scharowski KR, Nissen L, Lorenz D, Koch G, Ziegler M.
    Journal: Acta Histochem; 1976; 56(2):270-84. PubMed ID: 826097.
    Abstract:
    Insulin, glucagon and gastrin were investigated immunohistochemically in the islets of Langerhans in 14, 15, 17, 18 and 20 days old embryos, in newborn, 2, 5, 10, 20 and 30 days old and adult Wistar rats. The results were documented in series of microphotos. By means of the indirected immunofluorescence technique the insulin and glucagon were observed from the 18th day of the prenatal development in the B- resp. A-cells of the islets. Than the intensity of the fluorescence shows a continual increase during the development. In the islets cells of the developing stages and adult rats can't immunohistochemically evidenced the gastrin. The important results were discussed from the standpoint of the development physiology.
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