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  • Title: [Demonstration of an endo-beta-N-acetyl-glucosaminidase in rabbit serum].
    Author: Delmotte F, Kiéda C, Monsigny M.
    Journal: C R Acad Hebd Seances Acad Sci D; 1976 Oct 18; 283(9):1121-4. PubMed ID: 826351.
    Abstract:
    An endo-beta-N-acetyl glucosaminidase of rabbit serum has been characterized after purification by affinity chromatography. The specificity was determined by fluorimetric monitoring of the hydrolysis of 4-methylumbelliferyl-beta-glycosides and by thin layer chromatography identification of the hydrolysis products of fluoresceinyl-beta-glycosides. This enzyme acts on the following oligosaccharides GlcNac-beta-1-4 leads to GlcNAc-beta-O-R giving free di-N-acetyl chitobiose and R-OH as hydrolytic products.
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