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  • Title: Downstream insertion of the adenovirus tripartite leader sequence enhances expression in universal eukaryotic vectors.
    Author: Sheay W, Nelson S, Martinez I, Chu TH, Bhatia S, Dornburg R.
    Journal: Biotechniques; 1993 Nov; 15(5):856-62. PubMed ID: 8267981.
    Abstract:
    A series of universal eukaryotic gene expression vectors was constructed. All vectors contain a viral promoter and enhancer, a polylinker for insertion of the gene of interest and a polyadenylation sequence. To enhance translation, we inserted the tripartite leader sequence of an adenovirus downstream of the promoter. Using the chloramphenicol acetyl transferase (cat) gene as a marker, we show that the strength of various promoters/enhancers in different cell lines differed by two orders of magnitude. The presence of the tripartite leader increased the efficiency of gene expression up to 18-fold. The level of increase is promoter specific and is most likely influenced by additional sequences flanking the tripartite leader sequence.
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