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  • Title: [Human thyroid gland DNA methylase in nodular and diffuse goiter].
    Author: Nikol'skaia II, Sharkova EV, Zenina EN, Lopatina NG, Atakhanova BA, Adylova AT, Koneeva AE, Kovalev LI, Shishkin SS.
    Journal: Biokhimiia; 1993 Oct; 58(10):1616-24. PubMed ID: 8268304.
    Abstract:
    The heterogeneity and some properties of DNA-methylases isolated from nonmalignant human thyroid formations--nodular and diffuse goiters--have been studied. Isoelectrofocusing of methylase preparations produced 6-7 distinct activity peaks distinguished by pI, activity towards Ca2+ and Mg2+, sensitivity towards dithiothreitol and capacity to methylase cytosine into mono-, di- and tripyrimidine blocks in vitro. The degree of DNA methylation in vivo depended on the origin of of the goiter. Analysis of several methylase fractions by two-dimensional electrophoresis performed according to O'Farrell revealed the presence of major polypeptides having similar molecular masses (approximately 36 kDa). Besides the major component, all the preparations under study contained 6-10 characteristic minor components. The data obtained are suggestive of a structural and functional heterogeneity of human methylases.
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