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  • Title: Ethanol effect on the ionic transfer through isolated human amnion. II. Cellular targets of the in vitro acute ethanol action and of the antagonism between magnesium, taurine and ethanol.
    Author: Guiet-Bara A, Bara M.
    Journal: Cell Mol Biol (Noisy-le-grand); 1993 Nov; 39(7):715-22. PubMed ID: 8268757.
    Abstract:
    The ultrastructure of the human amniotic membrane from normal pregnancies at term, was studied by transmission electron microscopy. The results were analysed by a stereological method which indicates the ratio between the area of intercellular spaces (R1), microvilli (R2) and podocytes (R3) versus the cell area. Addition of ethanol (0.4 g/l) in the external medium decreased R1 and R3 and had no significant effect on R2. Ethanol reduced intercellular space and podocyte exchange surface. Two mM MgCl2 or 2 mM taurine restored R1 and R3 to normal values. The cellular targets of the antagonism between Mg, taurine and ethanol are particularly intercellular spaces and podocytes.
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