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  • Title: Quantitative enzyme cytochemistry during human macrophage development.
    Author: Sokol RJ, Hudson G, Wales JM, Goldstein DJ, James NT.
    Journal: J Anat; 1993 Aug; 183 ( Pt 1)(Pt 1):97-101. PubMed ID: 8270480.
    Abstract:
    Integrating microdensitometry was used to study changes in the intracellular activity of 4 enzymes during macrophage development. Suspension cultures of blood monocytes from 19 healthy human subjects were examined at 0, 2, 4 and 6 d. Mononuclear phagocytes were harvested by glass adherence and standard methods were used for cytochemical staining for NADH dehydrogenase, succinate dehydrogenase, acid phosphatase and alpha-naphthyl butyrate esterase. All specimens from all subjects were stained at the same time and staining intensities in individual cells were measured at appropriate wavelengths. A highly significant increase in enzyme activity with culture time was found for all 4 enzymes. These increases in mitochondrial, lysosomal and ectoenzyme activities during development indicate the increasing functional capabilities of the macrophages.
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