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  • Title: Formation of dry-heat resistant Bacillus subtilis var. niger spores as influenced by the composition of the sporulation medium.
    Author: Molin G, Svensson M.
    Journal: Antonie Van Leeuwenhoek; 1976; 42(4):387-95. PubMed ID: 827974.
    Abstract:
    Bacillus subtilis var. niger spores were produced on 20 different media. The spore yield from each medium and the dry-heat resistance at 160 C of the different spore populations were determined. The yield varied with a factor of 10(6) and the variation in D 160-value was about 10-fold (less than 20 S-190 S). A "synthetic" medium producing a high yield of spores with high dry-heat resistance was formulated. The concentrations of glucose, sucrose and calcium were found to be critical.
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