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  • Title: Specific transcriptional activation in vitro by the herpes simplex virus protein VP16.
    Author: Arnosti DN, Preston CM, Hagmann M, Schaffner W, Hope RG, Laughlan G, Luisi BF.
    Journal: Nucleic Acids Res; 1993 Dec 11; 21(24):5570-6. PubMed ID: 8284200.
    Abstract:
    The herpes simplex virus protein VP16 interacts with cellular factors, including the protein Oct-1, to activate viral immediate early (IE) gene transcription. We have reproduced this effect by addition of purified, full-length VP16 and the DNA-binding 'POU' domain of Oct-1 (Oct-1/POU) to a HeLa cell in vitro transcription system. Stimulation of transcription was dependent on the IE-specific element, TAATGARAT. In agreement with earlier observations from electrophoretic mobility shift assays, activation was not observed when Oct-2/POU, the DNA-binding domain from the Oct-2 protein, was substituted for Oct-1/POU. Single round transcription assays revealed that, together, VP16 and Oct-1/POU facilitate the assembly of pre-initiation complexes at target gene promoters.
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