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  • Title: Site-specific labelling of the oligosaccharide chains of antibodies.
    Author: Schwarz U, Wunderlich G, Brossmer R.
    Journal: FEBS Lett; 1994 Jan 10; 337(2):213-6. PubMed ID: 8287979.
    Abstract:
    This paper presents a new method for site-specific labelling of antibodies employing enzymatic reactions without oxidizing or reducing agents. IgG was first treated with immobilized sialidase from Clostridium perfringens to cleave bound NeuAc. CMP-9-deoxy-9-salizoyl-NeuAc, an activated sialic acid analogue, was labelled with 131I via the iodogen-method in high yields (> 95%). Then the oligosaccharide chains of antibodies were labelled yield with the radioactive NeuAc analogue by transfer using alpha-2,6-sialyltransferase from rat liver in 50%.
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