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  • Title: Construction of a bovine genomic library of large yeast artificial chromosome clones.
    Author: Libert F, Lefort A, Okimoto R, Womack J, Georges M.
    Journal: Genomics; 1993 Nov; 18(2):270-6. PubMed ID: 8288229.
    Abstract:
    We have constructed a yeast artificial chromosome (YAC) library derived from bull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8 x 12 positions) and in 2 x 224 96-well microplates maintained as permanent frozen stocks. An average insert size of 750 kb was estimated from the analysis of 200 randomly selected YACs, giving a sixfold coverage of the bovine genome for the total library. A screening strategy based on the polymerase chain reaction that allows the identification of individual clones has been devised. Screening of the library with six locus-specific markers of interest led to the isolation of 34 positive clones. The proportion of chimeric YACs was estimated from the analysis of a subset of the positive clones: 3 of 9 YACs were found to contain noncontiguous DNA.
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