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  • Title: [Expression of the vif gene of human immunodeficiency virus type 1 in Escherichia coli and study of the immunoreactivity of the vif protein].
    Author: Karaseva NG, Gibadulin RA, Kiselev SI, Karamov EV.
    Journal: Mol Gen Mikrobiol Virusol; 1993; (5):26-9. PubMed ID: 8289843.
    Abstract:
    Full-sized gen vif of human immunodeficiency virus has been synthesized and cloned into plasmid pGEX-2T. Vif-gene expression was found in Escherichia coli cells resulting in production of a hybrid GST-protein. The recombinant protein studied by the immunoblotting technique reacted with 8 of 22 probes of human HIV-positive sera. The recombinant protein is specifically cut by thrombin in two proteins corresponding to GST and VIF-proteins in molecular mass.
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