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  • Title: [Sorbent for purification of diamine oxidase by the method of affinity chromatography].
    Author: Klimova GI, Gromova LN, Gorkin VZ.
    Journal: Prikl Biokhim Mikrobiol; 1976; 12(4):614-8. PubMed ID: 829158.
    Abstract:
    A substrate of diamine oxidase hexamethylene diamine was covalently bound through adipinic acid dihydrazide to Sepharose 4B in order to prepare a sorbent for the purification of diamine oxidase by means of biospecific (affinity) chromatography. A method was developed to purify diamine oxidase from pig kidney cortex using the sorbent. The method, comprising three steps, yielded enzyme preparations with specific activity 2 000-fold higher than that of kidney homogenate.
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