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  • Title: Introduction of antisense oligonucleotides into cells by permeabilization with streptolysin O.
    Author: Barry EL, Gesek FA, Friedman PA.
    Journal: Biotechniques; 1993 Dec; 15(6):1016-8, 1020. PubMed ID: 8292333.
    Abstract:
    Cellular uptake of antisense oligonucleotides is critical to their ability to inhibit gene expression. In the present study, phosphodiester oligodeoxynucleotides were introduced into cells during brief permeabilization with the pore-forming agent streptolysin O. The extent of antisense inhibition was dependent on the concentration of oligonucleotide present during permeabilization. In addition, the level of antisense inhibition was time-dependent; it reached a maximum at 18 h and subsequently diminished to control levels over the next 48 h. These results demonstrate the effectiveness of streptolysin O permeabilization as a means for simple and rapid introduction of oligonucleotides into eukaryotic cells.
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