These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Studies on the extraction of phospholipids from erythrocyte membranes in the rat.
    Author: Eder K, Reichlmayr-Lais AM, Kirchgessner M.
    Journal: Clin Chim Acta; 1993 Oct 15; 219(1-2):93-104. PubMed ID: 8306467.
    Abstract:
    The effects of choice of solvent and period of extraction on recovery of phospholipid classes extracted from rat erythrocyte membranes were investigated. For extraction of phosphatidylserine (PS) the best recovery was achieved using chloroform-methanol (2:1, v/v), for diacyl phosphatidylethanolamine (PE) acetone-isopropanol (3:2, v/v), and for extraction of plasmalogen PE, phosphatidylcholine (PC) and sphingomyelin (SM) using hexane-isopropanol (3:2, v/v). Using hexane-isopropanol (3:2, v/v), boiling during the extraction increased the recovery of some of the individual phospholipid classes whereas mechanical treatment of the sample had only a slight effect. Furthermore, the fatty acid composition of PS and PE varied most with different extraction conditions and the phospholipid classes PC and SM were extracted more readily from rat erythrocyte membranes than PS, diacyl and plasmalogen PE.
    [Abstract] [Full Text] [Related] [New Search]