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  • Title: [Some properties of "soluble" Na+ and K+-ATPase obtained from various subcellular membrane structures of the brain by use of non-ionic detergents].
    Author: Kravtsov OV, Kirsenko OV.
    Journal: Ukr Biokhim Zh; 1975; 47(1):44-8. PubMed ID: 831.
    Abstract:
    A comparative study was carried out of some properties of "soluble" Na+, K+-ATPase obtained from different subcellular membrane brain structures by means of non-ionic detergents of triton X-100 and digitonin. It is established that temperature and pH-optima of "soluble" Na+, K+-ATPase are close to these optima of the initial membrane preparations. A certain difference is observed in the dynamics of temperature and pH-dependence of Na+, K+-ATPase activity in the extracts from different subcellular structures. The stability of the preparations in storage was investigated. A conclusion is made that more stable enzyme extracts may be obtained by means of digitonin.
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