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  • Title: Suppression of the neoplastic phenotype in vivo by an anti-ras ribozyme.
    Author: Kashani-Sabet M, Funato T, Florenes VA, Fodstad O, Scanlon KJ.
    Journal: Cancer Res; 1994 Feb 15; 54(4):900-2. PubMed ID: 8313379.
    Abstract:
    In this study, the efficacy of an anti-ras ribozyme in reversing the neoplastic phenotype was investigated. Murine NIH3T3 cells were transfected with cellular DNA from the FEMX-I human melanoma cell line expressing the activated H-ras gene. The transformed cells displayed the neoplastic phenotype in vitro and were tumorigenic in nude mice in vivo. When the transformants were transfected by a ribozyme designed to cleave only activated H-ras RNA, the transformed phenotype was abrogated. In contrast, expression of a mutant ribozyme, essentially acting only as antisense, into the transformed cells resulted in less dramatic changes in cell growth and tumorigenicity. These results reinforce the potential role of anti-oncogene ribozymes as suppressors of neoplastic growth, with possible implications for gene therapy.
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