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  • Title: Direct fluorescent-antibody confirmation of chlamydial antigen below the detection threshold of the chlamydiazyme enzyme-linked immunosorbent assay.
    Author: Kellogg JA, Seiple JW, Stroll ES.
    Journal: J Clin Microbiol; 1993 Jun; 31(6):1646-7. PubMed ID: 8315010.
    Abstract:
    Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed with the direct fluorescent-antibody test (Syva) from among those 66 Chlamydiazyme-negative specimens which had A492s that ranged from 0.030 to the cutoff and that could be blocked by > or = 50% with the blocking reagent.
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