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Title: Antigenic analysis of Chlamydia pecorum and mammalian Chlamydia psittaci by use of monoclonal antibodies to the major outer membrane protein and a 56- to 64-kd protein. Author: Kuroda-Kitagawa Y, Suzuki-Muramatsu C, Yamaguchi T, Fukushi H, Hirai K. Journal: Am J Vet Res; 1993 May; 54(5):709-12. PubMed ID: 8317762. Abstract: Monoclonal antibodies were prepared against 40- and 56- to 64-kd antigens of Chlamydia pecorum strain Maeda, which was isolated from a cow with pneumonia. Using the monoclonal antibodies, 5 strains of C pecorum, 25 strains of mammalian and 19 strains of avian C psittaci, 1 strain of C pneumoniae, and 3 strains of C trachomatis were analyzed for immunologic reactivity by use of the indirect immunofluorescent test. Monoclonal antibody analysis revealed immunologic relatedness between C pecorum and mammalian strains of C psittaci, which were completely differentiated from the other avian strains. Bovine strains were distinguished from ovine strains. Antigenic diversity was observed for bovine and ovine strains. Feline- and guinea pig-derived strains were shown to be immunologically different from bovine and ovine strains. Results provide the basis for typing and epidemiologic study of bovine and ovine strains of C pecorum and C psittaci.[Abstract] [Full Text] [Related] [New Search]