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  • Title: High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases.
    Author: Zhao RH, Li BY, Chen N, Zhang YK, Wang ZY, Lu PC.
    Journal: Biomed Chromatogr; 1993; 7(3):139-42. PubMed ID: 8318830.
    Abstract:
    A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer-assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.
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