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  • Title: Complex formation between RAS and RAF and other protein kinases.
    Author: Van Aelst L, Barr M, Marcus S, Polverino A, Wigler M.
    Journal: Proc Natl Acad Sci U S A; 1993 Jul 01; 90(13):6213-7. PubMed ID: 8327501.
    Abstract:
    We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.
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