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  • Title: An interactive image processing system for the quantification of cardiac function in the embryonic chick.
    Author: Xu B, Magee M, Bruyere H.
    Journal: Biomed Sci Instrum; 1993; 29():207-25. PubMed ID: 8329593.
    Abstract:
    This paper describes an interactive computer-based method for quantifying embryonic chick heart blood pool volumes that have been recorded as digitized image sequences. The primary motivation for the development of such a system is to be able to extract measurement data that is useful for evaluating potential drug-induced effects on intracardiac blood flow while minimizing variabilities in the measurements that typically occur when human evaluators extract the measurements visually from photographs. The processing that is used to achieve this goal can be divided into four basic steps: enhancing the contrast of the digitized embryonic chick heart image, extracting the shape of the blood pool in the embryonic chick heart ventricle from the enhanced image, quantifying the axial dimensions of the blood pool and calculating the required cardiac blood flow data. The technical details and the basic theory motivating each processing step are provided as well as an analysis of the results obtained by applying the method to actual image data.
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