These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effects of polyamines on intracellular calcium and mechanical activity in smooth muscle of guinea-pig taenia coli.
    Author: Nilsson BO, Hellstrand P.
    Journal: Acta Physiol Scand; 1993 May; 148(1):37-43. PubMed ID: 8333295.
    Abstract:
    The effects of the polyamines putrescine, spermidine and spermine on the mechanical activity in smooth muscle from guinea-pig taenia coli were investigated. Tissue concentrations of spermidine and spermine were about 240 and 160 nmol g-1, respectively, while the putrescine concentration was much lower (4 nmol g-1). Intracellular [Ca2+] was determined using the Fura-2 method and the effects of extracellularly applied polyamines were observed. Spermine (10(-5)-10(-3) mol l-1) dose-dependently inhibited spontaneous contractions as well as contractions induced by 20-25 mmol l-1 KCl, with 10(-3) mol l-1 spermine giving nearly total relaxation. Spermidine (10(-3) mol l-1) relaxed a 20 mmol l-1 KCl contracture, while putrescine had no effect. A decrease in intracellular [Ca2+] accompanied the relaxation induced by spermine. In contrast to the effects on spontaneous activity and intermediate [KCl], both the contraction and the accompanying increase in intracellular [Ca2+] induced by 90-120 mmol l-1 KCl were unaffected by spermine. Spermine did not affect the maximal Ca(2+)-induced force of chemically skinned taenia coli preparations, but a small potentiating effect on the Ca2+ sensitivity was observed. The results indicate that the relaxation induced by spermine depends on an intact cell membrane. Furthermore, the relaxing effect is due to decreased intracellular [Ca2+] and seems to occur only when the contraction is elicited by repetitive action potentials, and not by sustained depolarization.
    [Abstract] [Full Text] [Related] [New Search]