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  • Title: Frequency of B cells expressing germ-line gamma 1 transcripts upon IL-4 induction.
    Author: Turaga PS, Berton MT, Teale JM.
    Journal: J Immunol; 1993 Aug 01; 151(3):1383-90. PubMed ID: 8335934.
    Abstract:
    Murine B cells when activated with LPS and IL-4 have been shown to secrete IgG1 and IgE with corresponding H chain gene rearrangements. Using this system it has been previously demonstrated that transcription of gamma 1 and epsilon C region genes in their germ-line configuration occurs before switch recombination. Here, for the first time, the frequencies of B cells expressing germ-line gamma 1 transcripts are analyzed using in situ hybridization methodology. The results indicate that the combination of LPS and IL-4 induces a relatively low frequency of cells (5-12%) to express germ-line gamma 1 transcripts. Germ-line gamma 1 transcript-expressing cells were first detected on day 1 of culture with LPS and IL-4 and reached a maximum by days 3 to 5. The B cells that expressed germ-line gamma 1 transcripts appeared to be activated based on size and morphology. The frequency of B cells expressing germ-line gamma 1 transcripts approximated the frequency of C gamma 1+ plasma cells that appeared later in the cultures. These data may suggest a correlation between germ-line Ig transcript expression and the frequency of B cell precursors committed to the expression of a particular isotype.
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